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Method and kit for the determination of blood plasma inorganic polyphosphate

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The UCA research group working on the “Phosphate metabolism in human tissues” has developed a method and kit for determining the presence of inorganic polyphosphates (polyP) in blood plasma. The procedure consists of the concentration of most of the polyP by means of cryoprecipitation of the proteins fraction of the plasma. This makes it a very simple method in which contamination by the free phosphate in the sample is considerably reduced.

Molécula de PolyP en proteínas

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Description

Recently diverse functions associated with polyP in the field of medicine have been described. Examples include the modulation of cellular proliferation, angiogenesis, bone mineralization, energy metabolism and metastasis of tumours, as well as the functions of polyP related to inflammation, and several other conditions.

The research group has described, inter alia, how polyP accumulates in human platelets and is secreted by the platelets activated when blood coagulates. The group has determined that polyP in its entirety binds to the total fraction of proteins, and this allows the elimination of interference from the free phosphates in the plasma, which do not adhere to proteins. The method comprises the following steps: 1. obtaining the biological sample; 2. separating the fraction of proteins of high molecular weight (by the simple process of freezing and subsequently thawing the plasma, thus forming the cryoprecipitate); and 3. determining the content of polyphosphate within the fraction of proteins, which contains the greater part of the total polyphosphate.

Advantages

• This innovation will facilitate the diagnosis of patients who present blood coagulation alterations (such as platelet delta storage pool disease and Von Willebrand disease) and measurement of the polyphosphate content in the dense granules of the platelets by the new method devised.

• Studies have shown that polyphosphate values in blood may be related to diabetes. It is therefore hoped that the more in-depth study of polyP will lead to improvements in diagnosis of the disease, and that the method can be used as a tool to monitor the efficacy of diabetes treatments.

• This innovative method for the determination of polyP levels is both simple and inexpensive, thanks to the use of the cryoprecipitation technique.

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AGR (2)  BIO (1)  CTS (4)  FQM (5)  HUM (2)  RNM (12)  SEJ (1)  TEP (15)  TIC (5) 

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